Coding
Gli1-vioC

Part:BBa_K323086

Designed by: Tina Lebar, Tjaša Stošicki   Group: iGEM10_Slovenia   (2010-10-20)

Gli1_vioC

This part is composited from the Gli1 zinc finger gene(Part:BBa_K165007) and the vioC gene, which codes for the last enzyme in the violacein biosynthetic pathway - an FAD dependent monooxygenase, which hydroxylates the product of the VioD enzyme to violacein. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioC enzyme, which binds to a specific DNA sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1869
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None